Journal: Journal of Biological Chemistry
Article Title: AKR1A1 is a novel mammalian S-nitroso-glutathione reductase
doi: 10.1074/jbc.ra119.011067
Figure Lengend Snippet: Figure 2. Purification of NADPH-dependent GSNO reductase activity. (A) Representative Coomassie-stained SDS-PAGE gel corresponding to the chromatographic purification scheme of NADPH-dependent GSNO reductase activity (described in Table 1), which yielded a highly purified species from a crude kidney extract (lane 1) that was identified as aldo-keto reductase 1A1 (AKR1A1) (lane 7) (see also Fig. S1, Table S1).
Article Snippet: Chloroform was used to extract the aqueous phase and RNA from this phase was purified using the Aurum purification kit (BioRad) following manufacturer’s instructions.
Techniques: Purification, Activity Assay, Staining, SDS Page